Oil body proteins sequentially accumulate throughout seed development in Brassica napus |
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Authors: | Jolivet Pascale Boulard Céline Bellamy Annick Valot Benoît d'Andréa Sabine Zivy Michel Nesi Nathalie Chardot Thierry |
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Institution: | a INRA, AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, F-78000 Versailles, France b INRA, Amélioration des Plantes et Biotechnologies Végétales, UMR 118, F-35650 Le Rheu, France c INRA, CNRS, Plateforme de Protéomique, UMR 320, Ferme du Moulon, F-91190 Gif-sur-Yvette, France |
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Abstract: | Despite the importance of seed oil bodies (OBs) as enclosed compartments for oil storage, little is known about lipid and protein accumulation in OBs during seed formation. OBs from rapeseed (Brassica napus) consist of a triacylglycerol (TAG) core surrounded by a phospholipid monolayer embedded with integral proteins which confer high stability to OBs in the mature dry seed. In the present study, we investigated lipid and protein accumulation patterns throughout seed development (from 5 to 65 days after pollination DAP]) both in the whole seed and in purified OBs. Deposition of the major proteins (oleosins, caleosins and steroleosins) into OBs was assessed through (i) gene expression pattern, (ii) proteomics analysis, and (iii) protein immunodetection. For the first time, a sequential deposition of integral OB proteins was established. Accumulation of oleosins and caleosins was observed starting from early stages of seed development (12-17 DAP), while steroleosins accumulated later (∼25 DAP) onwards. |
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Keywords: | DAP days after pollination ER endoplasmic reticulum FA fatty acid hd hydrodynamic diameter OB oil body TAG triacylglycerol |
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