| 白血病抑制因子对胚泡金属蛋白酶表达的影响 |
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| 引用本文： | 孔英,葛常辉,燕秋,朱正美.白血病抑制因子对胚泡金属蛋白酶表达的影响[J].生物化学与生物物理进展,2002,29(2):278-282. |
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| 作者姓名： | 孔英 葛常辉 燕秋 朱正美 |
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| 作者单位： | 大连医科大学生物化学教研室,大连,116027 |
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| 基金项目： | 国家自然科学基金资助项目（39870184）. |
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| 摘 要： | 为了探讨白血病抑制因子 (LIF) 对胚泡着床作用的机理，胚泡经与LIF及其特异性抗体培养后，通过RT-PCR及免疫印迹技术，分析了LIF与着床前小鼠胚泡的基质金属蛋白酶9(MMP9)表达和分泌之间的关系.结果显示：LIF可明显诱导胚泡MMP9的分泌和基因表达； 经LIF特异性抗体封闭后，胚泡MMP9的分泌及基因表达下降，且下降趋势随着LIF被封闭时间延长而减弱，对MMPs组织抑制因子1(TIMP1)的影响则不明显.说明LIF可能通过诱导MMP9的分泌及基因表达来影响胚泡对子宫内膜细胞外基质的水解，促进着床.
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| 关 键 词： | 小鼠，胚泡，白血病抑制因子，基质金属蛋白酶，单克隆抗体 |
| 收稿时间： | 2001/7/23 0:00:00 |
| 修稿时间： | 2001年7月23日 |
Effect of LIF on Expression of Matrix Metalloproteinases (MMPs) in Cultured Mouse Embryo |
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| KONG Ying,GE Chang-Hui,YAN Qiu and ZHU Zheng-Mei.Effect of LIF on Expression of Matrix Metalloproteinases (MMPs) in Cultured Mouse Embryo[J].Progress In Biochemistry and Biophysics,2002,29(2):278-282. |
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| Authors: | KONG Ying GE Chang-Hui YAN Qiu and ZHU Zheng-Mei |
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| Institution: | Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116027, China;Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116027, China;Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116027, China;Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116027, China |
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| Abstract: | In order to investigate the mechanism of the essential role of leukemia inhibitory factor (LIF) in blastocyst implantation, blastocyst was incubated with LIF and its specific antibody. The effect of LIF on matrix metalloproteinases (MMPs) of pre-implantation blastocyst was analysized by RT-PCR and immuno-bloting techniques. The results showed that gene expression and secretion of MMP9 were significantly induced by LIF treatment, after blocking by LIF antibody, those effects were decreased, and the decreasing tendency was weakened with the increase of the incubation time. However the effect of LIF on tissue inhibitor of metalloproteinase1(TIMP1) was not obviously. The results indicate that LIF may play its role by inducing the gene expression and increasing the secretion of MMP9 to facilitate blastocyst implantation. |
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| Keywords: | mouse embryo leukemia inhibitory factor matrix metalloproteinases monoclonal antibody |
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