Different effects of 5-fluorouracil on Methanosarcina barkeri and on Methanobacterium thermoautotrophicum |
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| Authors: | Andrejs Grinbergs ,Volker Mü ller,Gerhard Gottschalk,Rudolf K. Thauer |
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| Affiliation: | Institut für Mikrobiologie, Universität Göttingen, Grisebachstr. 8, D-3400 Göttingen, F.R.G.;Mikrobiologie, Fachbereich Biologie, Philipps-Universität, Karl-von-Frisch-Strasse, D-3550 Marburg, F.R.G. |
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| Abstract: | Abstract Growth of Methanosarcina barkeri (strain Fusaro) was found to be inhibited by 5-fluorouracil (FU) only at relatively high concentrations (>50 μg / ml ). Inhibition could not be relieved by uracil. Therefore, FU probably did not exert its effect via inhibition of DNA synthesis as is the case in other organisms. Control experiments with Methanobacterium thermoautotrophicum (strain Marburg) on the other hand revealed that the effect of FU on this archaebacterium is probably exerted at the level of nucleic acid synthesis. The M. thermoautotrophicum cultures rapidly acquired resistance towards the pyramidine analog. |
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| Keywords: | Methanogenic bacteria Archaebacteria 5-Fluorouracil Salvage pathway Cell wall synthesis Autolysis Pyrimidine metabolism |
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