Containment of a genetically engineered microorganism during a field bioremediation application |
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Authors: | C Z Ford G S Sayler R S Burlage |
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Institution: | (1) Plant and Soil Science Department, Alabama A&M University, Normal, Alabama, USA, US;(2) Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA, US;(3) Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6036, USA e-mail: RVX@ORNL.GOV Tel.: +1-423-574-7321 Fax: +1-423-576-8543, US |
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Abstract: | A field release of a genetically engineered microorganism was performed at the Field Lysimeter Site on the Oak Ridge Reservation.
Six large lysimeters were filled with soil that had been contaminated with a mixture of naphthalene, phenanthrene, and anthracene.
A genetically engineered bacterial strain, Pseudomonas fluorescens HK44, was sprayed onto the surface of the soil during soil loading. This strain contains a fusion between the lux genes of Vibrio fischeri and the promoter for the lower pathway of naphthalene degradation, enabling the strain to become bioluminescent when it is
degrading naphthalene. Release of the bacteria outside the lysimeters was monitored, using selective agar plates and one-stage
Anderson air samplers. Although approximately 1014 bacteria were sprayed during the loading process, escape was only detected sporadically; the highest incidence of bacterial
escape was found when the relative humidity and wind speed were low.
Received: 6 March 1998 /thinsp;Received revision: 16 September 1998 / Accepted: 16 October 1998 |
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