Binding of a desmetallo-porphyrin conjugate of Hoechst 33258 to DNA. III. Strong bonding to single-strand oligonucleotides |
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| Authors: | Bichenkova E V Frau S Fedorova O S Douglas K T |
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| Affiliation: | School of Pharmacy and Pharmaceutical Sciences, University of Manchester, UK. |
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| Abstract: | The binding of the conjugate of Hoechst 33258 with 5,10,15,20-tetrakis (1-methyl-4-pyridyl)-21H,23H-porphyrin (PORHOE) to single-strand DNA has been detected by UV-vis spectrophotometry and 1H-NMR. The red-shift of porphyrin Soret band with strong hypochromicity indicates that the porphyrin moiety dominates in the interaction of the PORHOE with ssDNA. The affinity constants of PORHOE for d(GCATACAATTCG) or d(CGAATTGTATGC) were determined to be >10(5) M(-1), with strong cooperativity. |
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