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15N-marked aphids for predation studies under field conditions
Authors:K.M. Nienstedt and H.M. Poehling
Affiliation:(1) Institute of Plant Diseases and Plant Protection, University of Hanover, Herrenhäuser Str. 2, 30419 Hannover, Germany;(2) Present address: Springborn Laboratories (Europe) AG, Seestr. 21, CH-9326 Horn, Switzerland
Abstract:Individuals ofSitobion avenae (F.) (Homoptera: Aphididae) were marked with the stable isotope 15N through feeding on cereal seedlings which were immersed in a15N-enriched nutrient solution. Increased 15N-contents were obtained in all aphids with no detrimental effect on survival and fertility. When 15N-marked aphids were transferred to non 15N-enriched seedlings, after a short initial decreasing phase, 15N-contents remained nearly constant in time. A content of 10% 15N in the nutrient solution of the seedlings was sufficient for marking the aphids.
Keywords:15N  marker  aphids  Sitobion avenae
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