Immunity and resistance to the KP6 toxin of Ustilago maydis |
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Authors: | Aliza Finkler Tsafrira Peery Jianshi Tao Jeremy Bruenn and igal Koltin |
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Institution: | (1) Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Ramat Aviv, Israel;(2) Department of Biological Sciences, State University of New York at Buffalo, 14260 Buffalo, NY, USA |
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Abstract: | Summary The KP6 toxin of Ustilago maydis, encoded by segmented double-stranded (ds) RNA viruses, is lethal to sensitive strains of the same species and related species. The toxin consists of two polypeptides, and , synthesized as a single preprotoxin, which are not covalently linked. Neither polypeptide alone is toxic, but killer activity can be restored by in vitro and in vivo complementation. Killer-secreting strains are resistant to the toxin they produce. Resistance is conferred by a single recessive nuclear gene. This study describes a search for cytoplasmic factors that may confer resistance, also referred to as immunity. The approaches used to detect cytoplasmic immunity included transmission of dsRNA and transmission of virus particles to sensitive cells by cytoduction, cytoplasmic mixing in diploids and infection with viruses. An alternative approach was also used to express cloned cDNAs of the KP6 toxin-encoding dsRNA and of the and polypeptides. The results indicated that no immunity to KP6 can be detected. While KP6, and polypeptides were expressed by resistant cells, neither KP6 nor were expressed in sensitive strains. The polypeptide was expressed in sensitive cells, but it did not confer immunity. These results suggest that neither the preprotoxin nor the or polypeptides confer immunity and thus may be the toxic component of the binary toxin. |
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Keywords: | Ustilago maydis killer toxin Double-stranded RNA virus Immunity Transfection Expression vector |
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