Glycosphingolipids in feces of germ-free rats as a source for studies of developmental changes of intestinal epithelial cell surface carbohydrates |
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| Authors: | Göran Larson Tore Midtvedt |
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| Affiliation: | (1) Department of Clinical Chemistry, Gothenburg University, Sahlgren's Hospital, S-413 45 Gothenburg, Sweden;(2) Department of Medical Microbial Ecology, Karolinska Institute, S-104 01 Stockholm, Sweden |
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| Abstract: | Non-acid and acid glycosphingolipids were isolated from feces of one litter of germ-free rats from day 17 to day 51. Quantitative and qualitative changes described for small intestine of conventional rats [Bouhours D, Bouhours J-F (1981) Biochem Biophys Res Commun 99:1384–89] were also found in the feces of these germ-free rats. A decrease in lactosylceramide and sialyllactosylceramide excretion and a change fromN-acetylneuraminic acid toN-glycoloylneuraminic acid, as well as an appearance of type 1 chain blood group H-active penta- and decaglycosylceramides were observed during the weaning period. Thus the dramatic changes seen in rat intestinal glycosphingolipids postnatally seem to be primarily regulated by non-microbial factors.Abbreviations GM3 GM3-ganglioside, II3NeuAc-LacCer or II3NeuGc-LacCer - SPG IV3NeuAc-nLcOse4Cer - GM1 GM1-ganglioside, II3NeuAc-GgOse4Cer |
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| Keywords: | glycosphingolipids germ-free rats feces cell surface development |
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