首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Specific DNA duplex formation at an artificial lipid bilayer: towards a new DNA biosensor technology
Authors:Werz Emma  Korneev Sergei  Montilla-Martinez Malayko  Wagner Richard  Hemmler Roland  Walter Claudius  Eisfeld Jörg  Gall Karsten  Rosemeyer Helmut
Institution:Organic Chemistry I - Bioorganic Chemistry, Institute of Chemistry, University of Osnabrück, Barbarastrasse 7, D-49069 Osnabrück.
Abstract:A novel technique is described which comprises a base-specific DNA duplex formation at a lipid bilayer-H(2) O-phase boundary layer. Two different probes of oligonucleotides both carrying a double-tailed lipid at the 5'-terminus were incorporated into stable artificial lipid bilayers separating two compartments (cis/trans-channel) of an optically transparent microfluidic sample carrier with perfusion capabilities. Both the cis- and trans-channels are filled with saline buffer. Injection of a cyanine-5-labeled target DNA sequence, which is complementary to only one of the oligonucleotide probes, into the cis-channel, followed by a thorough perfusion, leads to an immobilization of the labeled complementary oligonucleotide on the membrane as detected by single-molecule fluorescence spectroscopy and microscopy. In the case of fluorescent but non-complementary DNA sequences, no immobilized fluorescent oligonucleotide duplex could be detected on the membrane. This clearly verifies a specific duplex formation at the membrane interface.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号