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Regeneration of Arabidopsis callus in vitro
Authors:Gregoria N. Acedo
Affiliation:(1) Department of Agronomy, University of Missouri, 65211 Columbia, MO, USA;(2) Present address: Environmental Health Research and Testing (EHRT), P.O. Box 12199, 27709 Research Triangle Park, N.C., USA
Abstract:This paper reports on an easy and reproducible method of regenerating Arabidopsis plants from callus culture. A combination of 6-benzylaminopurine (BAP) and agr-naphthalene acetic acid (NAA) in a Murashige and Skoog's (MS) based medium gives a high percentage of shoot formation in several genotypes.
Keywords:Arabidopsis  regeneration  callus culture  growth regulator
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