Development and characterization of novel tetra-, tri- and di-nucleotide microsatellite markers in cacao (<Emphasis Type="Italic">Theobroma cacao</Emphasis> L.) |
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Authors: | Ioná S Araújo Aline C Intorne Messias G Pereira Uilson V Lopes Gonçalo A de Souza Filho |
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Institution: | (1) Laboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28013-600, Brazil;(2) Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28013-600, Brazil;(3) Centro de Pesquisas do Cacau (CEPEC)/CEPLAC, Caixa Postal 7, Itabuna, BA, 45600-000, Brazil |
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Abstract: | The cacao plant, Theobroma cacao L., produces white seeds (beans) that form the major ingredient of processed chocolate. A great deal of research effort has
been expended to the development of new genetically modified cacao plants with improved productivity and resistance and beans
of good industrial quality. The availability of suitable genetic markers is an important aspect of the efficient selection
and breeding of this perennial species. We describe the development of 123 microsatellite loci of cacao. An optimized protocol
was used to construct and screen a microsatellite-enriched genomic library from which we isolated 64 di-nucleotide, 45 tri-nucleotide
and 14 tetra-nucleotide microsatellite loci. The primers were tested on samples from five different T. cacao accessions, one accession from T. grandiflorum and one accession from Herranea sp. Among the 123 loci, 54 were polymorphic, 61 were monomorphic and eight did not present an amplification product. These new
markers will be useful in future studies by increasing the accuracy of genotypic assessments in diverse cocoa tree populations
as well as in other species of the Theobroma genus. |
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Keywords: | Microsatellite Molecular markers Primers Theobroma |
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