Studies on in vitro colony formation by mouse bone-marrow cells using different sources of colony-stimulating factor |
| |
Authors: | Shellhaas James L Rheins Melvin S Filppi Joyce A |
| |
Institution: | (1) Present address: Department of Microbiology and Immunology, University of Louisville, 40232 Kentucky, Louisville;(2) Department of Microbiology, The Ohio State University, 484 W. 12th Avenue, 43210 Columbus, Ohio |
| |
Abstract: | Summary Conditioned media of a primary mouse embryo and a mouse cell line were compared as sources of colony-stimulating factor. The
incorporation of embryo cell conditioned medium into semisolid cultures of mouse bone-marrow cells induced the formation of
a larger number of in vitro colonies than did the addition of equal volumes of LM cell conditioned medium. This finding did
not appear to be the result of quantitative differences in the levels of C.S.F. between the sources since concentration of
the LM cell conditioned preparation did not enhance effectively the number of colonies produced. Both the colonial morphology
and the cellular components of the developing colonies were found to differ in accordance with the C.S.F. source employed
for stimulation. Colonies that developed under the influence of embryo cell conditioned medium were typically larger and more
disperse than were those produced in cultures stimulated with the LM cell conditioned source. The latter colonies were smaller,
more compact and contained fewer cells. Differences also were noted in the relative proportion of granulocytes to mononuclear
cells comprising the colonies. Those colonies stimulated with LM cell conditioned medium rapidly underwent a transition from
primarily a granulocytic composition to one comprised principally of mononuclear cells. Cultures stimulated with embryo cell
conditioned medium contained a greater number of granulocytic colonies which persisted for a protracted period during cultivation.
The addition of 2-mercaptoethanol to cultures stimulated with embryo cell conditioned medium increased the number of colonies
produced. Such synergy did not occur in cultures stimulated with the LM cell conditioned medium.
Supported by United States Public Health Service Research Grant CA 13752. |
| |
Keywords: | colony-stimulating factor in vitro colonies embryo cell conditioned LM cell conditioned |
本文献已被 SpringerLink 等数据库收录! |