Mutational analysis of primary alcohol metabolism in the methylotrophic actinomycete Amycolatopsis methanolica |
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| Authors: | Harm J Hektor Lubbert Dijkhuizen |
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| Institution: | Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands |
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| Abstract: | Abstract Mutants of the methylotrophic actinomycete Amycolatopsis methanolica unable to grow on methanol as carbon source were isolated and characterized. Mutants specifically affected in methanol utilization were deficient in formaldehyde assimilation. Mutants blocked in the first step of primary alcohol oxidation (C1–C4) had lost activity of the tetrazolium-dependent alcohol dehydrogenase, a three-component enzyme complex. This complex, or individual components, thus play a crucial role in utilization of primary alcohols in A. methanolica . |
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| Keywords: | Actinomycete Amycolatopsis methanolica Primary alcohol Ethanol Methanol Mutant Mutagenesis |
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