Effects of ziram on tumor-cell-binding capacity,cell-surface marker expression,and ATP levels of human natural killer cells |
| |
Authors: | Thyneice R Taylor Margaret M Whalen |
| |
Institution: | (1) Department of Biological Sciences, Tennessee State University, Nashville, TN 37209, USA;(2) Department of Chemistry, Tennessee State University, 3500 John A. Merritt Blvd., Nashville, TN 37209, USA; |
| |
Abstract: | Human natural killer (NK) cells are central in immune defense against tumor and virally infected cells. Ziram is used as an
accelerating agent in latex production and as an agricultural fungicide. Previous studies showed that continuous exposure
to ziram inhibits NK lytic function. Additionally, they showed that a brief (1 h) exposure to ziram caused persistent loss
of lytic function. This study examined whether decreases in lytic function were accompanied by decreases in the target-binding
function of NK cells and found that some, but not all, exposures to ziram caused significant decreases in binding function.
Ziram exposures that caused a loss of binding function were examined for effects on expression of key NK cell-surface proteins
needed for binding to targets. Exposure to 2 μM ziram for 1 h followed by 24 or 48 h in ziram-free media decreased CD16 expression,
but no other exposures caused decreases in cell-surface proteins. As decreases in adenosine triphosphate (ATP) could be in
part responsible for loss of lytic function, the effect of ziram exposures on ATP levels of NK cells were examined. Certain
ziram exposures decreased ATP levels in NK cells, but a decrease in ATP was not necessarily associated with a decrease in
lytic function. The results indicate that ziram-induced losses of lytic function cannot be fully explained by alteration in
binding, cell-surface protein expression, or ATP levels |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|