Cell transfection by DNA-lipid complexes — Lipoplexes |
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Authors: | Y S Tarahovsky |
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Institution: | 1.Institute of Theoretical and Experimental Biophysics,Russian Academy of Sciences,Pushchino, Moscow Region,Russia |
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Abstract: | Nonviral vectors such as complexes of plasmid DNA with cationic lipids known as lipoplexes are considered as an attractive
alternative to virus-based delivery systems. Unlike viruses, lipoplexes do not suffer from immunological and mutational hazards,
though the efficiency of lipoplexes is often not sufficient for therapeutic purposes and require higher level of transfection
than achieved until now. A number of critical steps responsible for transfection efficiency are discussed here. They include
processes of lipoplexes formation, interaction with cell surface, their internalization into cell, and DNA release and delivery
into the nucleus. All these processes should be thoroughly studied to be able to enhance the transfection efficacy. |
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