Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress |
| |
| Authors: | Efrén Santos Serge Remy Els Thiry Saskia Windelinckx Rony Swennen and László Sági |
| |
| Institution: | (1) Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 13, B-3001, Leuven, Belgium;(2) Current address: Centro de Investigaciones Biotecnol?gicas del Ecuador, Escuela Superior Polit?cnica del Litoral (ESPOL), Campus Gustavo Galindo, Km. 30.5 v?a Perimetral, Apartado, 09-01-5863 Guayaquil, Ecuador |
| |
| Abstract: | Background Next-generation transgenic plants will require a more precise regulation of transgene expression, preferably under the control
of native promoters. A genome-wide T-DNA tagging strategy was therefore performed for the identification and characterization
of novel banana promoters. Embryogenic cell suspensions of a plantain-type banana were transformed with a promoterless, codon-optimized
luciferase (luc
+) gene and low temperature-responsive luciferase activation was monitored in real time. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
