a Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-61265, Brno, Czech Republic
b Department of Pharmaceutical Chemistry, University of Bari, I-70125, Bari, Italy
Abstract:
In the present work, we measured survival and the platinum on the genome after treatment of repair-proficient or repair-deficient Escherichia coli strains with trans-PtCl2(E-iminoether)2] and compared these results with the effects of “classical” cisplatin. We found that toxicity of antitumor trans-PtCl2(E-iminoether)2] in repair-deficient trains was much less than that of cisplatin. This markedly reduced toxicity was not a consequence of the reduced uptake or low levels of DNA binding in the bacteria cells but rather appeared to reflect DNA binding mode of this trans-platinum drug different from that of cisplatin.