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Perfusion of the dog tibia isolated for the study of mineral metabolism |
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| Authors: | M Babé P Esbrit M L Traba C Castilla A Rapado |
| Abstract: | A method for perfusion of isolated canine tibia is described. With this procedure the influence of different albumins on the uptake of tritiated 25-hydroxyvitamin D3 (3H-25(OH)D3) by the tibia has been studied. Bovine serum albumin at 1% concentration seems to be optimum for this system. This experimental approach seems to be useful for the study of mineral metabolism. |
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