两种病毒侵染中国对虾后细胞超微病理学变化与免疫标记

应用电子显微技术研究人工感染的中国对虾病毒病原及其宿主细胞超微病理学变化。结果显示病虾体内存在球状与杆状两种病毒病原，有时在同一病虾组织的同一细胞中可见两种病毒同时侵染现象，该现象提示存在复合感染的可能。利用胶体金免疫标记技术对感染病虾细胞质中出现的球状病毒作定位标记，初步结果表明已分离提纯的球状病毒与感染病虾细胞质中观察到的病毒粒子性质基本相同。病毒侵染后，细胞内主要的细胞器如线粒体、内质网、核糖体均发生了显著变化；侵染后期，可见溶酶体及多种膜性结构大量增生、细胞核被一些微管样结构包裹等特殊变化的发生。

Cytopathic Changes of Penaeus chinensis infected by Two Kinds of Viruses and Immunogold Labelling

With electron microscopy techniques the major virus pathogens of artificially infected Penaeus chinensis were studied. It showed that there are two kinds of virus pathogens in diseased shrimps: spherical virus and baculovirus. Sometimes they were co - existing in the same tissue or even in the same cell. It suggested that a mixed infection maybe occurred. Using immunogold labelling method to label the spherical viruses appearing in the cytoplasm of diseased shrimp, preliminary results showed that the purified spherical viruses were the same as those observed in the infected cells. During the infection, prominent changes took place in main cell organelles of the host cell, such as mitochondrias, endoplasmic reticulum, and ribosome. Especially in late infection, a lot of lysosomes and various multilayered membrane structures were found, some nuclei were wrapped up by some microtubule structures.