| 免疫荧光检测HIV—1抗体方法的建立和应用 |
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| 引用本文： | 李敬云 王宏霞. 免疫荧光检测HIV—1抗体方法的建立和应用[J]. Virologica Sinica, 1997, 12(4): 371-373 |
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| 作者姓名： | 李敬云 王宏霞 |
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| 作者单位： | 北京军事医学科学院微生物流行病研究所!北京，100071，北京军事医学科学院微生物流行病研究所!北京，100071，北京军事医学科学院微生物流行病研究所!北京，100071 |
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| 摘 要： | 免疫荧光法检测HIV抗体是确认HIV感染的方法之一［l］，与蛋白印迹法相比，它的特异性更高，易于鉴别非特异反应，更为经济、快速、容易操作。为了拥有更多的HIV抗体检测手段，我们用感染和未感染HIV－l的HeLaCD“细胞制备抗原片，建立了免疫荧光检测HIV抗体的方法，并进行了初步应用。材料和方法1细胞和病毒HeLaCD4”细胞，引自美国BruceChesebro博士的实验室，用含10％热灭活小牛血清的RPMI1640培养基培养和传代。HIV－l株引自美国。2血清艾滋诊断试剂国家参比品，批号9602Z16份经蛋白印迹确认的HIV抗体阳性血清；10份健康…
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| 关 键 词： | 免疫荧光 HIV－1 HeLaCD_4~ 细胞 |
The Establishment and Application of Immunofluorescence Assay for Detection of Antibodies to HIV-1 |
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| Li Jingyun, Wang Hongxia, Bao Zuoyi. The Establishment and Application of Immunofluorescence Assay for Detection of Antibodies to HIV-1[J]. 中国病毒学(英文版), 1997, 12(4): 371-373 |
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| Authors: | Li Jingyun Wang Hongxia Bao Zuoyi |
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| Abstract: | Using the mixture of HIV -1 infected and no - infected HeLa CD4 cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of HIV antibody reagents were tested. The sensitivity and specificity were 96. 4 %and 100%, respectively. Compared with the confirmation test of Western Blot, the coincident rate was 92. 6 %. The results indicated that the specificity of immunofluorescence assay was high, and the positive predicted value was 100%, so it can be used as one of the confirmatory methods for HIV -1 antibody. |
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| Keywords: | Immunofluorescence assay HIV - 1 HeLa CD4~ cells |
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