Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor |
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| Authors: | Zhong Joan Q Freyzon Yelena Ehrlich Daniel J Matsudaira Paul |
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| Affiliation: | Whitehead Institute for Biomedical Research, MIT, Nine Cambridge Center, Cambridge, MA 02142, USA. |
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| Abstract: | We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein-protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface. |
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