Molecular cloning and characterization of antimicrobial peptides from skin of the broad-folded frog, Hylarana latouchii |
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Authors: | Wang Hui Yu Zhijun Hu Yuhong Yu Haining Ran Ran Xia Jiangnan Wang Duo Yang Shujie Yang Xiaohong Liu Jingze |
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Institution: | Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 20 Nanerhuan Dong Road, Shijiazhuang, Hebei 050016, China. |
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Abstract: | Seven cDNA sequences encoding antimicrobial peptide (AMP) precursors were cloned by screening the skin-derived cDNA library of the broad-folded frog, Hylarana latouchii. Seven of the deduced peptides are highly similar to AMPs in five families of brevinin-2 (brevinin-2LTa, brevinin-2LTb, and brevinin-2LTc), esculentin-1 (esculentin-1LTa), esculentin-2 (esculentin-2LTa), palustrin-2 (palustrin-2LTa), and temporin (temporin-LTe). The actual sequences and characteristics of mature AMPs were analyzed by RP-HPLC and LC–MS/MS-based proteomics approaches in combination of four different protein digestion processes and by LTQ XL in combination of gas-phase fractionation (GPF) analysis. Moreover, most of the peptides found in this study hardly display hemolytic activity in vitro, suggesting they are promising antimicrobial drug candidates. |
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Keywords: | Amphibian Hylarana latouchii Frog skin Antimicrobial peptide (AMP) |
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