白桦CESA7基因启动子的克隆与表达分析

克隆得到了一个白桦纤维素合成酶基因（CESA7）GenBanK登录号（EU591531）启动子序列，通过序列分析发现该启动子含有多个不同功能的顺式作用元件，包括光响应元件、激素响应元件、叶片形态发育元件等，推测该启动子在白桦生长发育过程中具有关键作用。将BpCESA7启动子克隆至带有GUS报告基因的植物表达载体，命名为proBpCESA7-121-GUS，并利用农杆菌介导方法侵染白桦和拟南芥，然后通过GUS组织化学染色观察BpCESA7基因启动子的组织表达特性。结果在白桦的根、茎、叶和拟南芥的根，叶，萼片、雌蕊中检测到了GUS活性，说明BpCESA7基因启动子具有启动子活性，并且在白桦的根和叶中染色最深，表明BpCESA7基因在白桦根和叶中表达量较高，并且其存在组织表达特异性。

Cloning and Expression Analysis of Promoter of CESA7 Gene in Betula platyphylla

A cellulosic synthase gene Betula platyphylla(CESA7) GenBank ID(EU591531) promoter was cloned and analyzed. By sequence analysis, the promoter was found to contain multiple cis-acting elements with different functions, including response elements, hormone-responsive elements, leaf morphology development elements, etc. It was speculated that this promoter plays a key role in the growth and development of birch. We cloned the promoter of BpCESA7 into a plant expression vector with a GUS reporter gene, named proBpCESA7-121-GUS, and invaded B.platyphylla and Arabidopsis thaliana by Agrobacterium-mediated method, and then observed that BpCESA7 gene was activated by GUS histochemical staining. Sub-organizational expression characteristics. Results GUS activity was detected in the roots, stems and leaves of B.platyphylla and Arabidopsis roots, leaves, sepals, and stamens, indicating that the promoter of BpCESA7 gene had promoter activity and was stained the most in the roots and leaves of birch, indicating BpCESA7 genes are highly expressed in the birch roots and leaves, and their tissue-specific expression is present.