碳源、生长素及诱导子对木豆不定根生长和次生代谢产物合成的影响

以1/2MS为基本培养基，研究了蔗糖、IBA、茉莉酸甲酯（MJ）及水杨酸（SA）对木豆不定根生物量和次生代谢产物合成的影响。研究结果表明，蔗糖浓度对木豆不定根的生物量和次生代谢产物合成有显著影响，蔗糖浓度为30 g·L^-1时，木豆不定根的生物量和次生代谢产物的含量均达到最大值。低浓度的IBA有利于木豆不定根的生长和次生代谢产物的积累，高浓度的IBA表现出抑制作用。IBA浓度为0.1 mg·L^-1时，生物量、染料木素及芹菜素含量均为最大值，分别为对照组的1.1、1.1和2.8倍。在0~200 μmol·L^-1浓度范围内，MJ对不定根的生长几乎无影响（P>0.05），但对次生代谢产物的合成有重要影响。MJ浓度为100 μmol·L^-1时，染料木素和芹菜素的含量均达到最大值，分别为对照组的1.9和2.1倍。SA抑制木豆不定根的生长和染料木素的合成，但对芹菜素的合成有一定促进作用，SA浓度为100 μmol·L^-1时，芹菜素的含量最高，为对照组的1.5倍。木豆不定根的悬浮培养是获得次生代谢产物的一条有效途径，为大规模生产染料木素和芹菜素提供了很好的思路。

Effect of Carbon Source,Auxin and Elicitor on the Growth and Synthesis of Secondary Metabolites of Adventitious Roots of Cajanus cajan(Linn.) Millsp.

Using 1/2MS as basic medium, we studied sucrose, IBA, jasmonic acid methylester(MJ) and salicylic acid(SA) on the influence of biomass and synthesis of secondary metabolites of adventitious root of Cajanus cajan(Linn.) Millsp.. The content of sucrose had a significant influence on the biomass and synthesis of secondary metabolites. When the content of sucrose was 30 g·L^-1, the amount of biomass and the content of secondary metabolites of adventitious root of C. cajan(Linn.) Millsp were the maximum value. The low concentration of IBA is beneficial to the growth of adventitious root of C. cajan(Linn.) Millsp. and the accumulation of secondary metabolites. However, the high content of IBA shows inhibition. When the content of IBA was 0.1 mg·L^-1, biomass, genistein and apigenin were maximum, 1.1, 1.1 and 2.8 times as control group respectively. MJ with 0-200 μmol·L^-1 almost had no effect on adventitious roots growth(P>0.05), but it had a significant effect on the synthesis of secondary metabolites. When the content of MJ was 100 μmol·L^-1, genistein and apigenin were maximum, respectively, 1.9 and 2.1 times as the control group. The growth of adventitious roots and the synthesis of genistein were inhibited by SA. However, SA had a certain effect on the synthesis of apigenin. When the content of SA was 100 μmol·L^-1, the content of apigenin was maximum, 1.5 times as the control group. Suspension culture of adventitious roots of C. cajan(Linn.) was an effective method to obtain the secondary metabolites, and will provide a good way of thinking for large-scale production of genistein and apigenin.