短瓣金莲花种子无菌萌发与幼苗快繁技术初探

以野生短瓣金莲花种子为材料，探讨不同处理方式对种子发芽的影响，并比较不同培养基类型及激素组合对短瓣金莲花根茎部位形成的愈伤组织、不定芽增殖及生长的影响，建立无菌播种及组培快繁体系，为短瓣金莲花快繁和规模化生产提供技术支撑。结果表明：（1）用600 mg·L^-1 GA₃溶液浸泡48 h后并用0.1% HgCl₂溶液灭菌20 min的种子发芽效果最好；（2）适宜于增殖的培养基配方为MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA；（3）适宜于生根的培养基配方为不添加激素的MS。

Tissue Culture and Rapid Propagation of Trollius ledebouri

The seeds of Trollius ledebouri Reichb. were used by different concentration and treating time of GA₃ to promote germination, and their buds were inoculated on different types of medium with different hormone. A rapid propagation system by tissue culture was established and become basis for large-scale production of T.ledebouri. The results showed that:(1)the seeds of T.ledebouri Reichb was efficiently sterilized by 0.1% HgCl₂ for 20 min after soaking in 600 mg·L^-1 GA₃ solution for 48 h and the germination was high; (2)the suitable medium for multiplication of T.ledebouri was MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA; (3)the suitable rooting medium was MS without any hormone.