Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction |
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Authors: | Takahashi Yusuke Yoshida Akihiro Nagayoshi Masato Kitamura Chiaki Nishihara Tatsuji Awano Shuji Ansai Toshihiro |
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Institution: | Division of Community Oral Health Science, Kyushu Dental College, Kokurakita-ku, Kitakyushu 803-8580, Japan. |
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Abstract: | To discriminate between viable and non-viable Enterococcus faecalis, the predominant pathogen in apical periodontitis, a real-time PCR method combined with propidium monoazide (PMA) was developed and evaluated. PMA had no antimicrobial effect on E. faecalis cells and permitted enumeration of both viable and non-viable cells. Therefore, E. faecalis cells from the root canals of nine patients with apical periodontitis were analyzed to evaluate the diagnostic usefulness of this approach. Viable and non-viable E. faecalis cells were successfully discriminated in these clinical specimens. A real-time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis. |
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Keywords: | apical periodontitis Enterococcus faecalis propidium monoazide viable cell |
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