Intact tissue assay for nitrite reductase in barley aleurone layers |
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| Authors: | Ferrari T E Varner J E |
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| Affiliation: | Department of Horticulture and Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823. |
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| Abstract: | A method has been devised for the detection and measurement of nitrite reductase activity in intact barley (Hordeum vulgare L. cv. Himalaya) aleurone layers. The technique involves feeding aleurone layers nitrite and measuring nitrite disappearance after a given time period. The method also allows simultaneous determination of nitrite uptake by the tissue. Quantitative recovery of nitrite is obtained by rapid heating of tissue in the presence of dimethyl sulfoxide. |
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