Cloning and characterization of the crystal protein-encoding gene of Bacillus thuringiensis subsp. yunnanensis. |
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| Authors: | Periasamy Balasubramanian R Jayakumar Prashant Shambharkar N Unnamalai S Karutha Pandian N Selvamuthu Kumaraswami R Ilangovan Vaithilingam Sekar |
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| Institution: | Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India. |
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| Abstract: | Molecular cloning and characterization of a novel cry gene, cry32Aa, of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutella xylostella. |
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