银离子抗性细菌质粒的分离与鉴定

通过对不同化工厂废水处理池的活性污泥进行富集、不同浓度银离子驯化后，分离得到30株银离子抗性细菌，最大硝酸银耐受量可达80 mg·ml^-1.对这些细菌进行质粒抽提与鉴定，质粒检出率为76.67％.40 mmol·L^-1的苯甲酸钠对HAg4细菌质粒的消除率可达98.75％；而350 μg· ml^-1的吖啶橙对HAg4细菌质粒的消除率只有77.78％.细菌质粒与银离子抗性密切相关.

Isolation and determination of silver-resistant bacteria plasmids

Through the enrichment of the active mud obtained from three chemical plants and the domestication with different concentration Ag+ solution, thirty bacteria strains with silver (Ag+)-resistance were isolated, among which, the highest Ag+ -resistant concentration was 80 mg x ml(-1). The plasmids in these bacteria were extracted, with the detection rate of 76.67%. The elimination rate of the plasmid in HAg4 bacteria was 98.75% by 40 mmol x L(-1) sodium benzoate, and 77.78% by 350 microg x ml(-1) acridine orange. It was suggested that the Ag+ -resistance of bacteria was highly correlated with their plasmids.