Incorporation of phytol precursors into chlorophylls of tobacco cell cultures |
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Authors: | Jürgen Benz Ulrika Lempert Wolfhart Rüdiger |
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Institution: | (1) Botanisches Institut der Universität, Menzinger Strasse 67, D-8000 München 19, Germany |
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Abstract: | The incorporation of 1-3H] geranylgeranyl diphosphate (GGPP), 1-3H] geranylgeranyl monophosphate (GGMP) and U-14C] phytyl diphosphate (PhPP) into chlorophylls a and b in growing tobacco cell cultures was investigated. The substrates were effectively incorporated into chlorophylls a and b, 3.2% of the total activity of applied GGPP or GGMP and 12.4% of the total activity of applied PhPP being found in chlorophylls a and b after 24 h incubation. The radioactivity was found in phytyl chlorophyllide through-out which means effective hydrogenation of the alcohol moiety in the case of GGPP and GGMP. With increasing substrate concentration, the specific radioactivity of chlorophyll increased up to a saturation level which was reached either at 20–40 M PhPP or at 60 M GGPP and GGMP. The specific radioactivity of the chlorophyll formed during the 24-h incubation period was the same as that of the applied substrate at saturating substrate concentration. The specific radioactivity of chlorophyll a was higher than that of chlorophyll b only in the case of PhPP.Abbreviations Chlide
chlorophyllide a
- ChlPh
phytyl chloro-phyllide
- ChlGG
geranylgeranyl chlorophyllide a
- GGPP
geranylgeranyl diphosphate
- GGMP
geranylgeranyl monophosphate
- HPLC
high-performance liquid chromatography
- PhPP
phytyl diphosphate
Dedicated to Professor Dr. Hubert Ziegler on the occasion of his 60th birthday |
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Keywords: | Chlorophyll biosynthesis Nicotiana (chlorophyll biosynthesis) Geranylgeranyl phosphate Phytol |
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