枯草芽孢杆菌微生态制剂制备及在仿刺参养殖中的应用

采用2%海藻酸钠为壁材,4%氯化钙作为固定化剂,将发酵后收集的枯草芽孢杆菌菌体制备成微生态制剂。将该枯草芽孢杆菌微生态制剂添加到仿刺参基础饲料中,对仿刺参进行喂养,观察其对仿刺参消化酶活性以及生长和仿刺参养殖水质的影响。试验组在基础饲料上添加5 mL的枯草芽孢杆菌微生态制剂,其投喂量为仿刺参体重的5%,并且换水周期为3 d。试验结果表明,添加枯草芽孢杆菌微生态制剂的试验组水质中亚硝酸盐、氨氮、化学需氧量均低于对照组。通过2个生长阶段的比较,试验组内仿刺参肠道内淀粉酶、蛋白酶和纤维素酶活性均高于对照组,尤其淀粉酶活力提高明显。通过对试验组和对照组中仿刺参的质量增加率和质量日增重量进行比较,在生长第1个阶段里两组数值差距不明显,但在生长第2个阶段里,两组数值差距逐渐增大,这说明微生态制荆对仿刺参生长的影响,随着时间逐渐增大。

Preparation of Bacillus subtilis microbial ecological agent and its application to sea cucumber culture

Using 2% sodium alginate as wall material and 4% CaCl2 as immobilized agent, the fermented Bacillus subtilis as microbial eeologieal agent was prepared to feed the sea cucumbers by mixing the Bacillus probioties, and then to observe the effects on the digestive enzyme activity of the sea eueumbers as well as the impact of the water quality where they were fanned. In the experiment, the sea eueumbers with 5 ml Bacillus subtilis were fed and the water was changed in every three days. The results showed that the amounts of nitrite, ammonia nitrogen and chemical oxygen in the experimental water were detected lower than those of the control group. By comparing two growth stages, the activities of amylase, protease and eellulase in the sea cueumber gut of the test group were evaluated; especially the amylase activity was signifieantly increased. By comparing the increasing rate of the sea eucumbers＇ mass quantity and quality of daily gain between the test group and control group, the conclusion was that in the first stage of growth there was not obvious difference, but in the second stage the gap of data between the two sets gradually increased. It illustrated that the effects of Bacillus subtilis on the growth of sea cucumbers gradually increase with time.