| 人核糖体DNA打靶载体介导mda-7基因对肝癌的体外基因治疗研究 |
| |
| 引用本文： | 薛金锋,刘雄昊,何嫱,薛志刚,胡友金,李卓,杨俊林,高庭,潘乾,龙志高,邬玲仟,夏昆,梁德生,夏家辉.人核糖体DNA打靶载体介导mda-7基因对肝癌的体外基因治疗研究[J].生物化学与生物物理进展,2009,36(11):1429-1435. |
| |
| 作者姓名： | 薛金锋 刘雄昊 何嫱 薛志刚 胡友金 李卓 杨俊林 高庭 潘乾 龙志高 邬玲仟 夏昆 梁德生 夏家辉 |
| |
| 作者单位： | 中南大学医学遗传学国家重点实验室,长沙,410078 |
| |
| 基金项目： | 国家重点基础研究发展计划(973)(2004CB518800)，国家高技术研究发展计划(863)(2007AA021002, 2007AA021206)和国家自然科学基金(30700458, 30971298) 资助项目 |
| |
| 摘 要： | 人核糖体DNA打靶载体pHr是一种由中南大学医学遗传学国家重点实验室开发构建的针对人类基因组的同源重组质粒载体．利用pHr构建了一种mda-7/GFP融合基因的人源基因表达载体pHr-CMG，并研究了其在肝癌细胞系Bel-7402中的作用．利用荧光显微镜、RT-PCR和Western blotting检测了mda-7/GFP融合基因的表达；利用细胞周期分析、MTT和Hoechst33258染色研究了其在细胞中的作用．结果显示，pHr-CMG载体能在Bel-7402细胞中有效表达MDA-7/GFP融合蛋白，进而抑制细胞增殖和诱导细胞凋亡，推测其可能是由载体表达了mda-7基因引起细胞在G2/M期累积所导致的．同时，实验结果证实了人核糖体DNA打靶载体系统以及pHr-CMG表达载体的有效性，为其在进一步基因治疗研究中的应用提供了理论和实验基础．
|
| 关 键 词： | 人核糖体DNA打靶载体，mda-7基因，肝癌，基因治疗 |
| 收稿时间： | 2009/4/21 0:00:00 |
| 修稿时间： | 2009/6/26 0:00:00 |
In vitro Efficacy of mda-7 Gene for Hepatocellular Carcinoma Gene Therapy Mediated by Human Ribosomal DNA Targeting Vector |
| |
| XUE Jin-Feng,LIU Xiong-Hao,HE Qiang,XUE Zhi-Gang,HU You-Jin,LI Zhuo,YANG Jun-Ling,GAO Ting,PAN Qian,LONG Zhi-Gao,WU Ling-Qian,XIA Kun,LIANG De-Sheng and XIA Jia-Hui.In vitro Efficacy of mda-7 Gene for Hepatocellular Carcinoma Gene Therapy Mediated by Human Ribosomal DNA Targeting Vector[J].Progress In Biochemistry and Biophysics,2009,36(11):1429-1435. |
| |
| Authors: | XUE Jin-Feng LIU Xiong-Hao HE Qiang XUE Zhi-Gang HU You-Jin LI Zhuo YANG Jun-Ling GAO Ting PAN Qian LONG Zhi-Gao WU Ling-Qian XIA Kun LIANG De-Sheng and XIA Jia-Hui |
| |
| Institution: | State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China;State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China |
| |
| Abstract: | Human ribosomal DNA (hrDNA) targeting vector pHr is a homologous recombinant plasmid for human genome which developed in the State Key Laboratory of Medical Genetics. pHr was used to construct a recombinant plasmid pHr-CMG expressing mda-7/GFP fusion gene and its efficacy in the hepatocellular carcinoma cell line Bel-7402 was investigated. The expression of mda-7/GFP fusion gene was detected by fluorescent microscope, RT-PCR and Western blotting, and its function was detected by cell-cycle analyses, MTT assay and Hoechst33258 staining. The results demonstrated that pHr-CMG vector could express MDA-7/GFP fusion protein effectually and the mda-7 gene could induce cell apoptosis and proliferation suppression in Bel-7402 cell line, which might be caused by the G2/M cell cycle arrest. These results also suggested that human ribosomal DNA targeting vector system and the pHr-CMG vector may be applied in further gene therapy researches for hepatocellular carcinoma. |
| |
| Keywords: | hrDNA targeting vector mda-7 gene hepatocellular carcinoma gene therapy |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
