饲料中添加辅酶Q10对吉富罗非鱼幼鱼生长、抗氧化能力和组织结构的影响

以含辅酶Q10(CoQ10)分别为0、40、80和120 mg/kg的4种饲料饲喂平均初始体重为(19.97±0.13) g的吉富罗非鱼(Oreochromis niloticus, GIFT)幼鱼56d,探讨辅酶Q10对吉富罗非鱼幼鱼生长性能、体成分、抗氧化能力、组织结构和基因表达的影响。结果显示,各辅酶Q10组吉富罗非鱼幼鱼的终末体重、摄食率、特定生长率和饲料效率与对照组均无显著差异, 120 mg/kg辅酶Q10组终末体重、特定生长率和饲料效率均为最高;辅酶Q10含量为120 mg/kg时,吉富罗非鱼幼鱼的干物质消化率显著升高;各辅酶Q10组血清过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性均显著高于对照组;各辅酶Q10组肝脏CAT和GSH-Px活性均显著高于对照组, 80和120 mg/kg辅酶Q10组肝脏总超氧化物歧化酶(SOD)和谷胱甘肽S转移酶(GST)活性均显著升高,丙二醛(MDA)含量显著降低;各实验组去内脏全鱼的水分、粗蛋白和灰分含量均无显著性差异, 120 mg/kg辅酶Q10组去内脏全鱼粗脂肪显著低于对照组;各实验组内脏团水分、粗蛋白、粗脂肪和灰分...

DIETARY COENZYME Q10 ON GROWTH,ANTIOXIDANT CAPACITY AND TISSUE STRUCTURE OF JUVENILE GIFT TILAPIA (OREOCHROMIS NILOTICUS)

To evaluate the effects of dietary coenzyme Q10 (CoQ10) on growth performance, body composition, antioxidant capacity, tissue structure and gene expression in juvenile genetically improved farmed tilapia (GIFT), juvenile GIFT tilapia with initial body weight of (19.97±0.13) g were fed with four diets containing 0, 40, 80 and 120 mg/kg CoQ10 for a 56-day trial. The results showed that the final body weight (FBW), feeding rate (FR), specific growth rate (SGR) and feed efficiency (FE) of supplemented CoQ10 groups had no significant difference compared with the control group, while 120 mg/kg CoQ10 group had the highest final body weight, specific growth rate and feed efficiency. The dry matter digestibility (DMD) of juvenile GIFT tilapia significantly increased when CoQ10 content was 120 mg/kg. The serum activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in CoQ10 groups were significantly higher than those in the control group. The CAT and GSH-Px activities in liver of the CoQ10 groups were significantly higher than those in the control group. The SOD and GST activities in liver of 80 and 120 mg/kg CoQ10 groups were significantly higher than those in control group while malondialdehyde (MDA) content was significantly decreased. There was no significant difference in the moisture, crude protein and ash contents in the eviscerated whole fish among all groups. Compared with the control group, 120 mg/kg CoQ10 significantly decreased crude lipid content in the eviscerated whole fish. There was no significant difference in the moisture, crude protein, crude lipid and ash contents in the visceral mass among all groups. No significant histological changes in liver were identified in all groups. The intestinal villi length, villi density and muscle thickness in the 120 mg/kg group significantly increased. The expression of sod, cat, gsh-px, gst and igm mRNA in 120 mg/kg CoQ10 group were significantly higher than the control group, while the expression of il-1β and il-8 mRNA significantly decreased in the CoQ10 groups compared with the control group. In the challenge experiment by intraperitoneal injection with Aeromonas hydrophila, the cumulative survival rate of juvenile GIFT tilapia in 80 and 120 mg/kg CoQ10 groups was significantly higher than that in control group. In conclusion, these results suggested that dietary CoQ10 content within 120 mg/kg has no adverse effects on juvenile GIFT tilapia, dietary 120 mg/kg CoQ10 could improve digestibility, antioxidant abilities, liver antioxidant-related gene expression and resistance to Aeromonas hydrophila of juvenile GIFT tilapia.