三疣梭子蟹卵巢发育过程中主要类胡萝卜素组成变化及其与抗氧化性能的关系

实验研究了三疣梭子蟹(Portunus trituberculatus)卵巢发育过程中(Ⅰ—V期)组织色泽、类胡萝卜素组成与含量、抗氧化和非特异性免疫指标的变化规律,进一步探讨了肝胰腺中类胡萝卜素含量变化与抗氧化指标的关系。结果表明:(1)卵巢发育期间,卵巢指数(GSI)显著增加(P<0.05),肝胰腺指数(HSI)整体呈先升后降的趋势;卵巢的红度(a*)值和黄度(b*)值、肝胰腺的亮度(L*)值和b*值均呈显著上升趋势,但卵巢中L*值呈下降趋势。(2)在卵巢发育过程中,卵巢中的总类胡萝卜素、虾青素、叶黄素和β-胡萝卜素含量均为先升后降的趋势,虾青素含量在Ⅳ期最高;肝胰腺中的虾青素含量呈上升趋势,而β-胡萝卜素含量为显著下降趋势;内表皮中的总类胡萝卜素、虾青素、叶黄素、海胆烯酮和β-胡萝卜素含量均呈现下降趋势;就不同组织中类胡萝卜素含量而言,内表皮中虾青素含量最高。卵巢和肝胰腺的L*值与总类胡萝卜素含量呈显著负相关,卵巢的a*值和b*值均与总类胡萝卜素、虾青素、叶黄素、海胆烯酮和β-胡萝卜素含量呈显著正相关。肝胰腺的a*值与各类胡萝卜素含量无显著相关性, b*值仅与虾青素含量呈显...

CHANGES IN CAROTENOID COMPOSITION AND ANTIOXIDANT CAPACITY OF PORTUNUS TRITUBERCULATUS DURING OVARIAN DEVELOPMENT

Female Portunus trituberculatus at different stages of ovarian development (stage Ⅰ—Ⅴ) were investigated for their color, carotenoids contents, antioxidant and non-specific immune parameters. The results indicated that: (1) the gonadosomatic index (GSI) increased significantly (P<0.05), while the hepatosomatic index (HSI) rose slightly, then declined gradually. The redness (a*) and yellowness (b*) values in ovaries showed an increasing trend, as did lightness (L*) and b* values in hepatopancreas, but L* values in ovaries showed a decreasing trend. (2) Total carotenoids, astaxanthin, zeaxanthin and β-carotene content in ovaries showed an increasing trend followed by a decreasing trend, with the highest astaxanthin content in stage IV. Astaxanthin content in hepatopancreas was on the rise, while β-carotene was on a significant decline. Regarding the content of carotenoids in different tissues, the epithelium contained the highest amount of astaxanthin. L* values in the ovary and hepatopancreas exhibited a significant negative correlation with total carotenoids, and a* and b* values in the ovary both showed a significant positive correlation with total carotenoids, astaxanthin, luteolin, echinenone and β-carotene. There was no significant correlation between a* values and various carotenoids in hepatopancreas, and b* values only showed a significant positive correlation with astaxanthin. (3) For antioxidant indexes, T-AOC in hepatopancreas showed a significant decrease in stage Ⅰ—Ⅴ (P<0.05), while SOD, CAT and GPX showed an “increasing and then decreasing” trend. The T-AOC in hemolymph showed a trend of “high-low-high-low”. SOD and GPX showed an increasing trend and then decreasing trend, while CAT and MDA showed a “low-high-low-high” trend. In terms of immune indexes, ACP, ALP and NO in the hepatopancreas showed an overall decreasing trend during ovarian development, while ACP, ALP and Hc in the hemolymph showed an increasing trend in stage Ⅰ—Ⅳ and a slight decrease in stage Ⅴ. (4) Correlation analysis showed that astaxanthin content in hepatopancreas was significantly negatively correlated with T-AOC, lutein content was significantly positively correlated with GPX (P<0.01), and no significant correlation was found between the other carotenoids content and antioxidant indexes. In conclusion, the increase of total carotenoids, astaxanthin and β-carotene in the ovaries of P. trituberculatus occurred mainly in stage Ⅱ—Ⅳ, while most of the carotenoids in the epithelium showed a significant decreasing trend and only astaxanthin in the hepatopancreas showed an increasing trend.