Pep5, a new lantibiotic: structural gene isolation and prepeptide sequence |
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Authors: | Cortina Kaletta Karl-Dieter Entian Roland Kellner Günther Jung Michaela Reis Hans-Georg Sahl |
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Affiliation: | (1) Institut für Mikrobiologie der Universität Frankfurt, D-6000 Frankfurt/M, Federal Republic of Germany;(2) Institut für Organische Chemie der Universität Tübingen, D-7400 Tübingen, Federal Republic of Germany;(3) Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, D-5300 Bonn, Federal Republic of Germany |
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Abstract: | A wobbled 14-mer oligonucleotide was derived from the amino acid sequence of the 34-residue propeptide of the lantibiotic Pep5 (Kellner et al. 1989). Using this hybridization probe, the structural gene of Pep5, pepA, was located on the 18.6 kbp plasmid pED503. The nucleotide sequence of pepA codes for a prepeptide with 60 residues and proves that Pep5 is ribosomally synthesized. The N-terminus of the prepeptide has a high -helix probability and a characteristic proteolytic cleavage site precedes the C-terminal 34-residue propeptide. Our present theory is that maturation of Pep5 involves (a) enzymic conversion of Thr, Ser and Cys into dehydrated amino acids and sulfide bridges, (b) membrane translocation and cleavage of the modified prepeptide.Dedicated to Prof. H. Zähner on the occasion of his sixtieth birthday |
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Keywords: | Lantibiotic Pep5 Nucleotide sequence Staphylococcus epidermidis 5 Lanthionine Non-proteinogenic amino acids Post-translational modification |
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