Chitosan antiviral activity: Dependence on structure and depolymerization method |
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Authors: | V N Davydova V P Nagorskaya V I Gorbach A A Kalitnik A V Reunov T F Solov’eva I M Ermak |
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Institution: | 1.Pacific Institute of Bioorganic Chemistry, Far East Division,Russian Academy of Sciences,Vladivostok,Russia |
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Abstract: | Enzymatic (the action of lysozyme) and chemical (the action of hydrogen peroxide) hydrolysis of chitosans with various degree
of acetylation (DA)—25, 17, and 1.5%—was performed. Purification and fractioning of the hydrolysis products were performed
using dialysis, ultrafiltration, and gel-penetrating chromatography. Low-molecular (LM) derivatives of the polysaccharide
with molecular masses from 17 to 2 kDa were obtained. The study of their antiviral activity against the tobacco mosaic virus
(TMV) showed that these samples inhibited the formation of local necroses induced by the virus for 50–90%. The antiviral activity
of the LM chitosans significantly increased with the lowering of their polymerization degree. Furthermore, the products of
the enzymatic hydrolysis possessed lower activity than the chitosan samples obtained as a result of chemical hydrolysis. It
was revealed that the exhibition of the antiviral activity weakly depended on the degree of acetylation of the samples. |
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