Ti plasmid type affects T-DNA processing in Agrobacterium tumefaciens |
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Authors: | Todd R Steck |
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Institution: | Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223, USA |
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Abstract: | Agrobacterium tumefaciens can transfer the T-DNA region of a Ti plasmid to a recipient plant cell. An accepted model that describes the T-DNA transfer mechanism proposes that single-stranded T-complexes are transferred to a recipient plant via a conjugation-like mechanism. This model has been based on examination of a limited number of Ti plasmids. In this study, the type of processed T-DNA molecule created from multiple Ti plasmids was determined. The form of the processed T-DNA was found to vary and was correlated with whether the T-DNA region was organized as a single continuous region or two adjacent regions. |
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Keywords: | Agrobacterium tumefaciens Ti plasmid T-DNA processing |
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