Increased hGM-CSF production and secretion with Pluronic F-68 in transgenicNicotiana tabacum suspension cell cultures |
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Authors: | Jong-Moon Cho Jun-Young Kwon Jung-Ae Lim Dong-Il Kim |
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Affiliation: | (1) Department of Biological Engineering, Inha University, 402-751 Incheon, Korea |
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Abstract: | The effects of Pluronic F-68, a nonionic surfactant, on the production and secretion of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) in a transgenicNicotiana tabacum cell suspension culture were investigated in this study. The addition of Pluronic F-68 was shown to extend cell survival in the stationary phase, but had no influence on effective initial cell growth. With regard to production, it increased the level of extracellular hGM-CSF two-fold. This may be attributable not only to the enhanced expression level, but also to the improved permeability of the cell membrane due to the interaction between Pluronic F-68 and the cell membrane and cell wall. The effect of Pluronic F-68 on the production and secretion of hGM-CSF in a bioreactor was also evaluated. hGM-CSF production in the bioreactor after the addition of Pluronic F-68 proved more effective than in flask cultures. |
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Keywords: | transgenic plant cell culture hGM-CSF Pluronic F-68 secretion permeabilization |
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