| 棉花大田植株叶柄组织培养体系的建立 |
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| 引用本文： | 张朝军,范术丽,武芝霞,张雪妍,王玉芬,李付广.棉花大田植株叶柄组织培养体系的建立[J].西北植物学报,2011,31(6). |
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| 作者姓名： | 张朝军 范术丽 武芝霞 张雪妍 王玉芬 李付广 |
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| 作者单位： | 中国农业科学院棉花研究所,农业部棉花遗传改良重点开放实验室,河南安阳455000 |
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| 基金项目： | "863"优质高产棉花分子品种创制 |
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| 摘 要： | 以早熟棉花品种'中棉所24'为材料,通过对大田棉花植株叶柄灭菌条件、叶柄愈伤组织诱导与分化、胚状体的萌发及植株再生所需的培养基等进行研究,以建立棉花大田植株叶柄组织培养体系.结果表明:棉花植株叶柄经0.1%HgCl2灭菌4～5 min后,横切成0.8 cm左右的小段,在MSB+0.05 mg·L-1IAA+0.05 mg·L-1KT+0.05 mg·L-12,4-D+25 g·L-1葡萄糖+2 g·L-1Gel(pH 5.8)培养基上培养,易获得分化的愈伤组织;愈伤组织在添加有KT/IAA(MSB+0.08 mg·L-1IAA+0.08 mg·L-1KT+25 g·L-1葡萄糖+2 g·L-1Gel,pH6.5)或ZT/IBA(MSB+0.1 mg·L-1ZT+0.1 mg·L-1IBA+25 g·L-1葡萄糖+2 g·L-1Gel,pH 6.5)的分化培养基中,均可以获得胚性愈伤组织.胚性愈伤组织在MSB+0.05 mg·L-16-BA+0.05 mg·L-1IAA+25 g·L-1蔗糖+2 g·L-1Gel(pH 6.5)培养基上,形成胚状体、生长发育成再生植株.
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| 关 键 词： | 棉花 叶柄 组织培养 |
Plantlets Regeneration from Mature Leaf's Petiole of Cotton |
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| ZHANG Chao-jun,FAN Shu-li,WU Zhi-xia,ZHANG Xue-yan,WANG Yu-fen,LI Fu-guang.Plantlets Regeneration from Mature Leaf's Petiole of Cotton[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(6). |
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| Authors: | ZHANG Chao-jun FAN Shu-li WU Zhi-xia ZHANG Xue-yan WANG Yu-fen LI Fu-guang |
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| Institution: | ZHANG Chao-jun,FAN Shu-li,WU Zhi-xia,ZHANG Xue-yan,WANG Yu-fen,LI Fu-guang(Cotton Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455002,China) |
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| Abstract: | The study was to stablish a tissue culture system with petioles of grown-in-field cotton plants as explants,using cotton variety CCRI 24 as materials by the analysis the sterilization conditions for petioles of grown-in-field cotton plants,the induction and differentiation of petiole callus,the culture medium used for embryoid germination and the plant regeneration.The results showed that sterilization requirement can be reached with petioles soaked in 0.1% HgCl2 for 4 to 5 minutes.Easily differentiated cal... |
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| Keywords: | cotton petiole tissue culture |
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