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| 同源四倍体灯盏花离体再生及其倍性鉴定 | |
| 引用本文： | 关文灵,李叶芳,郑思乡,李世峰.同源四倍体灯盏花离体再生及其倍性鉴定[J].西北植物学报,2011,31(6):1142-1146. |
| 作者姓名： | 关文灵 李叶芳 郑思乡 李世峰 |
| 作者单位： | 1. 云南农业大学园林园艺学院,昆明,650201 2. 贵州省农业科学院园艺研究所,贵阳,550006 3. 云南省农业科学院花卉研究所,昆明,650205 |
| 摘 要： | 以四倍体灯盏花的无菌苗叶柄为外植体进行离体培养,研究其再生体系,并对再生植株进行染色体倍性鉴定。结果表明:叶柄外植体在MS+0.05 mg·L-1NAA+0.5 mg·L-16-BA+0.65%琼脂+3%蔗糖的培养基上不定芽的再生频率可达87.3%;继代培养(MS+0.1 mg·L-1NAA+0.3 mg·L-16-BA+0.65%琼脂+3%蔗糖)增殖系数为7.8,诱导(1/2MS+1.0 mg·L-1NAA+1.0 mg·L-1IBA+0.65%琼脂+3%蔗糖)生根率100%,移栽成活率为90%。细胞学鉴定结果表明,再生植株的染色体数为2n=4x=36,而原二倍体的染色体数目为2n=2x=18,基数x=9,因此,再生植株为四倍体。 |
| 关 键 词： | 灯盏花 同源四倍体 叶柄 离体再生 |
Regeneration of Autotetraploid Erigeron breviscapus in vitro Culture and Identification of the Ploidy |
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| GUAN Wen-ling,LI Ye-fang,ZHENG Si-xiang,LI Shi-feng.Regeneration of Autotetraploid Erigeron breviscapus in vitro Culture and Identification of the Ploidy[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(6):1142-1146. | |
| Authors: | GUAN Wen-ling LI Ye-fang ZHENG Si-xiang LI Shi-feng |
| Institution: | GUAN Wen-ling1,LI Ye-fang1,ZHENG Si-xiang2,LI Shi-feng3(1 College of Landscape and Horticuture,Yunnan Agricultural University,Kunming 650201,China,2 Horticuture Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550006,3 Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China) |
| Abstract: | The system of adventitious shoot regeneration in autotetraploid Erigeron breviscapus in vitro was studied.Leafstalks from axenic autotetraploid E.breviscapus plantlets as explants were cultured on MS medium with different combinations of growth regulator,and the effects of growth regulator combinations on induction of shoots are evaluated.Lastly,the ploidy of regenerative plantlets was identified.The results indicated that the optimal medium for inducting adventitious shoots is MS media containing 0.65% aga... |
| Keywords: | Erigeron breviscapus autotetraploid leafstalk propagation in vitro |
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