The use of lignocellulosic wastes for production of cellulase by Trichoderma reesei |
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Authors: | R. Doppelbauer H. Esterbauer W. Steiner R. M. Lafferty H. Steinmüller |
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Affiliation: | (1) Institute of Biochemistry, University Graz, Austria;(2) Institute for Biotechnology, Microbiology and Waste Treatment, Technical University Graz, Austria;(3) Vöest Alpine AG, Linz, Austria;(4) Institute of Biochemistry, University of Graz, Schubertstr. 1, A-8010 Graz, Austria |
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Abstract: | Summary The feasibility of cellulase production by Trichoderma reesei using inexpensive lignocellulosic material was examined. Sulfite pulp used as standard substrate yielded 3.7 IU/ml filter paper units (FPU) and 2.15 IU/ml -glucosidase. The yield was 185 FPU per gram total carbohydrate (CH) in the fermentation medium. Steam treated wheat straw (2%) gave 1.9 FPU/ml, 0.83 IU/ml -glucosidase and 151 FPU/g CH, whereas the spent fibres remaining after enzymatic hydrolysis of steamed wheat straw gave 2.4 FPU/ml, 1.55 IU/ml -glucosidase and 147 FPU/g CH. A good substrate (3%) was also the combustible fraction of municipal waste (BRAM) treated with NaOH, which gave 2.5 FPU/ml, 0.86 IU/ml -glucosidase and 130 FPU/g CH. A further increase in the final enzyme titer is obtainable by increasing the substrate concentration. In shake cultures 5% steamed wheat straw gave 3.8 FPU/ml and 1.95 IU/ml -glucosidase. Untreated wheat straw gave only low final enzyme titers and low yields of FPU/g CH. In the case of lignocellulosic substrates a constant pH-value of pH 6.0 during the fermentation gave optimal yields. |
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