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半日花的组织培养及植株再生(简报)
引用本文:李晶,贾敬芬,郝建国.半日花的组织培养及植株再生(简报)[J].分子细胞生物学报,2003,36(4):318-322.
作者姓名:李晶  贾敬芬  郝建国
作者单位:西北大学生命科学学院,西北大学生命科学学院,西北大学生命科学学院 西安 710069,西安 710069,西安 710069
基金项目:国家自然科学基金(30070366)
摘    要:半日花(Helianthemum Songaricum Schrenk)是半日花科半日花属落叶小灌木,分布于新疆、甘肃和内蒙古等荒漠地区海拔1000米至1300米山地。为亚洲中部荒漠特有种和古地中海植物区系孑遗种,是生长了7000万年的远古植物。比具有“活化石”之称的水杉和银杏还要早几千万年。植物学家将它及与之同龄的四合木、沙冬青和革苞菊等十几种植物统称为“古老残遗濒危植物”。故而在研究亚洲中部荒漠,特别是研究我国荒漠植物区系的起源以及与地中海

关 键 词:半日花  组织培养  植株再生
修稿时间:2002年12月19

TISSUE CULTURE AND PLANT REGENERATION OF HELIANTHEMUM SONGARICUM SCHRENK
LI Jing JIA Jing fen HAO Jian guo.TISSUE CULTURE AND PLANT REGENERATION OF HELIANTHEMUM SONGARICUM SCHRENK[J].Journal of Molecular Cell Biology,2003,36(4):318-322.
Authors:LI Jing JIA Jing fen HAO Jian guo
Abstract:The cotyledonary segments of sterile seedlings of Helianthemum Songaricum Schrenk were cultured on different media containing different phytohormones. It was found that the calli could be induced efficiently on MS basal medium supplemented with 10. 0mg/L NAA and 0. 2mg/L 6-BA . When calli were transferred on MS medium with 2. 0mg/L 6-BA and 0. 2mg/L NAA, shoots were produced. The frequency of shoot differentiation reached about 85%. The regenerated shoots were rooted on 1 /2MS medium added with 0. 5mg/L NAA. The rooting rate was about 76% . Regenerated plantlets were successfully transplanted in soil, with a success rate of 67% .
Keywords:Helianthemum Songaricum Schrenk  Tissue culture  Plant regeneration  
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