| 高压液相色谱法分离寡糖的对氨基苯甲酸乙脂衍生物 |
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| 引用本文： | 蒋可,陈宇东,钟晋贤.高压液相色谱法分离寡糖的对氨基苯甲酸乙脂衍生物[J].中国生物化学与分子生物学报,1993,9(3):342-346. |
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| 作者姓名： | 蒋可 陈宇东 钟晋贤 |
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| 作者单位： | 中国科学院生态环境研究中心，中国科学院生态环境研究中心，中国科学院生态环境研究中心 北京 100085，北京 100085，北京 100085 |
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| 摘 要： | 寡糖的对氨基苯甲酸乙酯衍生物,以及它的全乙酰化产物具有紫外吸收基团适合HPLC分离,研究了氨基柱和十八烷基柱对寡糖混合物的分离性能及检测灵敏度。从免疫球蛋白M中分离到的寡糖组分,衍生后用HPLC进一步分离提纯出另一些含量较少的糖链。
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| 关 键 词： | 寡糖 高压液相色谱 免疫球蛋白M |
| 收稿时间： | 1993-06-20 |
Separation for Oligosacchrides with Ethyl p-aminobenzoate in Reducing Termini by High Performance Liquid Chromatography |
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| Jiang,Ke Chen,Yu-dong Zhong,Jin-xian.Separation for Oligosacchrides with Ethyl p-aminobenzoate in Reducing Termini by High Performance Liquid Chromatography[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(3):342-346. |
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| Authors: | Jiang Ke Chen Yu-dong Zhong Jin-xian |
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| Institution: | (Research center for Eco-environmental Sciences, Academia Sinica, Beijing 100085 |
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| Abstract: | A series of oligosacchrides were briefly derivatized the reducing termini with suitable UV-absorbing moiety (ABEE) and then all particular derivatives were applied to peracetylation, both with and without peracety]ation could be purified by HPLC. We compared with different results of resolution and sensitivity on an aminopropyl-bonded silicon column and C_(18) column. Using this experimental strategy a few minor components isolated from IgM were also obtained and detected. Improved sensitivity and resolution are especially useful for trace quantities of natural oligosacchrides. |
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| Keywords: | Oligosacchrides High Performance Liquid Chromatography Immunoglobuliu M |
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