Phosphorylation of brush border myosin at threonine on its 20 kDa light chains by a calmodulin-independent kinase activates its ATPase |
| |
| Authors: | C Borysenko J P Rieker H Swanljung-Collins J Montibeller J H Collins |
| |
| Affiliation: | Department of Microbiology, Biochemistry and Molecular Biology, School of Medicine, University of Pittsburgh, PA 15261. |
| |
| Abstract: | A calmodulin-independent kinase isolated from chicken intestinal brush border phosphorylates brush border myosin mainly at an apparently single threonine on its 20 kDa light chains. Phosphorylation to 1.9 mol phosphate/mol myosin activated the myosin actin-activated ATPase about 12-fold, to about 100 nmol/min per mg. Brush border myosin ATPase can thus be activated by phosphorylation either at threonine, by calmodulin-independent kinase, or at serine, by calmodulin-dependent myosin light chain kinase, as previously shown [(1987) FEBS Lett. 223, 262-266]. |
| |
| Keywords: | |
|
|
