| Heat shock induction of a 65 kDa ATP—binding proteinase in rat C6 glioma cells |
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| 引用本文： | XUCUNSHUAN MARCOMEYER 等.Heat shock induction of a 65 kDa ATP—binding proteinase in rat C6 glioma cells[J].细胞研究,1999,9(2):135-144. |
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| 作者姓名： | XUCUNSHUAN MARCOMEYER |
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| 作者单位： | [1]DepartmentofBiology,HenanNormalUniversity,Xinxiang453002 [2]InstituteofCellBiology,BremenUniversity,D－28 |
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| 摘 要： | The 45,55,65 and 100kDa ATP-binding proteinases(ATP-BPases) of the heat-shocked (44℃ for 30 min,recovery for 12h) rat C6 glioma cells were purified by DEAE-ionexchange and ATP-affinity chromatography.Their molecular masses,isoelectric points (pI),pH-optima and other properties were analyzed by native proteinase gels.It was shown that the 65 kDa ATP-BPase is specifically induced by heat shock and not detectable in control cells.Its N-terminal 1-9amino acid sequence was determined by Edman degradation,but no homologies to other proteins in the protein data bases were found.30 and 31kDa proteinases can be cleaved from the 45,55 and 65 kDa proteinases to which they are linked.A possible relationship of the heat-induced 65 kDa ATP-BPase with the ATP-dependent proteinases (ATP-DPases) in prokaryotes and eukaryotes is discussed.
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| 关 键 词： | 大鼠 C6神经胶质瘤细胞 热休克 诱导 65kDaATP结合蛋白酶 |
Heat shock induction of a 65 kDa ATP-binding proteinase in rat C6 glioma cells |
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| XU CUN SHUAN, WEI MING ZHANG, DIETER TECHEL, MARCO MEYER, YAN ZHANG LI,LUDGER RENSING.Heat shock induction of a 65 kDa ATP-binding proteinase in rat C6 glioma cells[J].Cell Research,1999,9(2):135-144. |
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| Authors: | XU CUN SHUAN WEI MING ZHANG DIETER TECHEL MARCO MEYER YAN ZHANG LI LUDGER RENSING |
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| Institution: | Department of Biology, Henan Normal University, Xinxiang. Peanut@public2.zz.ha.cn |
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| Abstract: | The 45, 55, 65 and 100 kDa ATP-binding proteinases (ATP-BPases) of the heat-shocked (44 degrees C for 30 min, recovery for 12 h) rat C6 glioma cells were purified by DEAE-ionexchange and ATP-affinity chromatography. Their molecular masses, isoelectric points (pI), pH-optima and other properties were analyzed by native proteinase gels. It was shown that the 65 kDa ATP-BPase is specifically induced by heat shock and not detectable in control cells. Its N-terminal 1-9 amino acid sequence was determined by Edman degradation, but no homologies to other proteins in the protein data bases were found. 30 and 31 kDa proteinases can be cleaved from the 45, 55 and 65 kDa proteinases to which they are linked. A possible relationship of the heat-induced 65 kDa ATP-BPase with the ATP-dependent proteinases (ATP-DPases) in prokaryotes and eukaryotes is discussed. |
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| Keywords: | Rat C6 glioma cells ATP-binding Proteinases heat shock induction native Proteinase gels |
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