The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgene |
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Authors: | So?Yeon?Yoo Kirsten?Bomblies Seung?Kwan?Yoo Jung?Won?Yang Mi?Suk?Choi Jong?Seob?Lee Detlef?Weigel Email author" target="_blank">Ji?Hoon?AhnEmail author |
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Institution: | (1) Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea;(2) School of Biological Sciences, Seoul National University, Seoul, 152-742, Korea;(3) Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany;(4) Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA;(5) School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea |
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Abstract: | Positive selection of transgenic plants is essential during plant transformation. Thus, strong promoters are often used in selectable marker genes to ensure successful selection. Many plant transformation vectors, including pPZP family vectors, use the 35S promoter as a regulatory sequence for their selectable marker genes. We found that the 35S promoter used in a selectable marker gene affected the expression pattern of a transgene, possibly leading to a misinterpretation of the result obtained from transgenic plants. It is likely that the 35S enhancer sequence in the 35S promoter is responsible for the interference, as in the activation tagging screen. This affected expression mostly disappeared in transgenic plants generated using vectors without the 35S sequences within their T-DNA region. Therefore, we suggest that caution should be used in selecting a plant transformation vector and in the interpretation of the results obtained from transgenic approaches using vectors carrying the 35S promoter sequences within their T-DNA regions. |
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Keywords: | 35S enhancer 35S promoter Misexpression Plant transformation vector Trans activation |
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