Gas chromatographic-mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil,sediment and biofilm |
| |
Authors: | Keinänen M M Korhonen L K Martikainen P J Vartiainen T Miettinen I T Lehtola M J Nenonen K Pajunen H Kontro M H |
| |
Institution: | Department of Environmental Health, National Public Health Institute, P.O. Box 95, FIN-70701, Kuopio, Finland. minna.keinanen@ktl.fi |
| |
Abstract: | Hydroxy fatty acids (OH-FAs) can be used in the characterization of microbial communities, especially Gram-negative bacteria. We prepared methyl esters of 2- and 3-OH-FAs from the lipid extraction residue of soil, sediment, and biofilm samples without further purification or derivatization of hydroxyl groups. OH-FA methyl esters were analyzed using a gas chromatograph equipped with a mass selective detector (GC-MS). The ions followed in MS were m/z 103 for 3-OH-FAs and m/z 90 and M-59 for 2-OH-FAs. The rapid determination of 3- and 2-OH-FAs concomitantly with phospholipid fatty acids provided more detailed information on the microbial communities present in soil, sediment, and drinking water biofilm. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|