| 博尔纳病病毒抗原免疫的杂交瘤细胞系的评价 |
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| 引用本文： | 贾丽娜,张凤民,宋武琦,李小光,钱钧,包丽丽,陈小贝,翟爱霞,张庆猛.博尔纳病病毒抗原免疫的杂交瘤细胞系的评价[J].中国微生态学杂志,2007,19(2):163-164,166. |
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| 作者姓名： | 贾丽娜 张凤民 宋武琦 李小光 钱钧 包丽丽 陈小贝 翟爱霞 张庆猛 |
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| 作者单位： | 哈尔滨医科大学,微生物学教研室,黑龙江省普通高校病原生物学重点实验室,黑龙江,哈尔滨,150086 |
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| 基金项目： | 国家自然科学基金;黑龙江省教育厅海外学人科研(合作)基金;黑龙江省教育厅骨干教师创新能力培养项目 |
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| 摘 要： | 目的评价博尔纳病病毒(BDV)抗原免疫的杂交瘤细胞系的产生抗体能力与抗体特异性。方法随机抽取2株由BDV抗原免疫的杂交瘤细胞系H1和H2,通过体内和体外方法制备单克隆抗体,通过间接免疫荧光、免疫印迹和间接酶联免疫吸附3种试验方法对制备的抗体进行特异性鉴定及效价测定。结果杂交瘤细胞系H1和H2可以产生一定效价的单克隆抗体,并对BDV的P24和P40抗原具有特异性。结论由杂交瘤细胞系H1和H2产生的单克隆抗体可以用于检测BDV特异性抗原。
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| 关 键 词： | 博尔纳病病毒 单克隆抗体 免疫荧光试验 免疫印迹试验 间接免疫吸附试验 |
| 文章编号： | 1005-376X（2007）02-0163-03 |
| 收稿时间： | 2006-10-31 |
| 修稿时间： | 2006-10-31 |
Identification of the hybridoma cell lines immunized by the antigens of Borna disease virus |
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| JIA Li-na,ZHANG Feng-min,SONG Wu-qi,LI Xiao-guang,QIAN Jun,Bao Li-li,CHEN Xiao-bei,ZHAI Ai-xia,ZHANG Qing-meng.Identification of the hybridoma cell lines immunized by the antigens of Borna disease virus[J].Chinese Journal of Microecology,2007,19(2):163-164,166. |
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| Authors: | JIA Li-na ZHANG Feng-min SONG Wu-qi LI Xiao-guang QIAN Jun Bao Li-li CHEN Xiao-bei ZHAI Ai-xia ZHANG Qing-meng |
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| Institution: | Department of Microbiology, Harbin Medical University, Key Lab of Pathogenic Biology, Universities of Heilongjiang Province, Harbin 150086, China |
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| Abstract: | Objective To identify the hybridoma cell lines immunized by the antigen of Borna disease virus(BDV).Methods Two hybridoma cell lines immunized with antigens of BDV were selected randomly and employed to produce the monoclonal antibodies(McAb) in vitro and in vivo.Then the titers and the specificity of the McAbs were determined by means of IFA,WB and ELISA method.Result The hybridoma cell lines could produce the McAbs which was specific to BDV P24 and P40 antigens with the appropriate titers.Conclusion The McAbs produced from the hybridoma cell lines are useful to detecting the BDV antigens. |
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| Keywords: | Brona disease Virus McAb IFA WB ELISA |
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