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Simultaneous measurement of changes in the membrane potential and the intracellular Ca2+ concentration caused by somatostatin in human GH-producing pituitary tumor cells.
Authors:N Yamashita  K Takano  A Teramoto  K Tatakura  E Ogata
Institution:Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.
Abstract:Changes in the membrane potential and the intracellular Ca2+ concentration (Ca2+]i) caused by somatostatin (SRIF) were simultaneously measured in human GH-producing pituitary tumor cells, by means of the nystatin-perforated whole cell clamp technique and Fura-2 AM. An application of 10(-8) M SRIF hyperpolarized the membrane and arrested Ca(2+)-dependent spontaneous action potentials. Ca2+]i concurrently decreased during membrane hyperpolarization. When the membrane potential was clamped below the threshold for voltage-gated Ca2+ channels, Ca2+]i decreased and SRIF did not further reduce Ca2+]i. In cells which did not show spontaneous action potentials, SRIF hyperpolarized the membrane but it affected Ca2+]i little. From these results it was concluded that the reduction in Ca2+]i caused by SRIF was ascribed to the decrease in Ca2+ influx through voltage-gated channels during membrane hyperpolarization. The effect of SRIF on the voltage-gated Ca2+ channel current was also examined under the perforated whole cell clamp. SRIF (10(-8) M) inhibited the Ca2+ channel current to 80.8 +/- 15.4% (n = 5) of the control. Because SRIF-induced inhibition of the voltage-gated Ca2+ channel current was not prominent, it was considered that membrane hyperpolarization is the major cause of the reduction in Ca2+]i in human GH-producing cells.
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