THE AFFINITY OF THE FUCOSE-BINDING LECTIN FROM LOTUS TETRAGONOLOBUS FOR GLYCOPEPTIDES AND OLIGOSACCHARIDES ACCUMULATING IN FUCOSIDOSIS |
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Authors: | Jean Philippe Susz Glyn Dawson |
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Institution: | Departments of Pediatrics and Biochemistry, Joseph P. Kennedy, Jr. Mental Retardation Research Center, University of Chicago, Pritzker School of Medicine, Chicago, IL 60637, U.S.A. |
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Abstract: | Abstract— The affinity of the fucose-binding lectin from Lotus tetragonolobus for fuco-oligosaccharides accumulating in the brain and other tissues of a patient with fucosidosis was studied by two methods: by inhibition of the co-precipitation of the lectin with porcine stomach mucin and by one-step affinity chromatography on a column of the lectin bound to Sepharose-4B. Both methods indicated that the lectin had greater affinity for the disaccharide Fuc(α, 1-6)GlcNAc than for either the main fucosidosis storage material in brain, a fuco-dekasaccharide, or the heterogeneous fuco-glycopeptide fractions obtained from normal human and rat brain glycoproteins. Our results suggest that the fucose residue linked α(1-6) to the N -acetylglucosamine residue involved in the N -glycosidic linkage to asparagine is not available to the lectin in the intact N -glycosidic chains of normal brain glycopeptide fractions and that the lectin has poor affinity for the Fuc(α, 1-3)Glc N Ac linkage in rat brain glycoproteins. |
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